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This situation invitations the design of C-2-substituted analogs of adenosine nucleotides and NAD+ as selective inhibitors of LigA. Indeed, it was noted recently that two-methylthio-ATP is really a strong inhibitor on the adenylylation response of E. coli
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S3), nonetheless the topology from the LigA clamp is starkly distinct from that from the clamps formed by ChVLig and HuLig1. The kissing contacts that shut the LigA clamp are sui generis
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